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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3A12.08
Gene ID 42/F05
Gene synonyms/obsolete
Gene product hypothetical protein; possibly fungal specific;
Entry clone Cloned
ORF length (unspliced) 645
ORF length (spliced)
Entry clone length 645
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC3A12.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTCTAAATTATTTCA
Rev primer name SPAC3A12.08.Rv
Rev primer SEQ AGAAAGCTGGGTACGATTGTAAATCCAAAGCT
Amino acid length 214
Molecular weight 23.9
Isoelectric point (calc.) 9.1
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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