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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP4H10.21c
Gene ID 42/F06
Gene synonyms/obsolete
Gene product GINS complex (predicted); involved in DNA replication (predicted)
Entry clone Cloned#
ORF length (unspliced) 645
ORF length (spliced)
Entry clone length 645
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP4H10.21.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGTGGGATGCTGATGA
Rev primer name SPBP4H10.21.Rv
Rev primer SEQ AGAAAGCTGGGTATATTAGTGAGACAACACCA
Amino acid length 214
Molecular weight 24.9
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol; a few nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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