| Gene name |
SPAC15E1.06 |
| Gene ID |
42/F04 |
| Gene synonyms/obsolete |
vps29 |
| Gene product |
metallophosphatase;
involved in intracellular protein transport; calcineurin-like
phosphoesterase; retromer complex; involved in retrograde
vesicle formation from the prevacuole |
| Entry clone |
Cloned |
| ORF length (unspliced) |
642 |
| ORF length (spliced) |
564 |
| Entry clone length |
642 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC15E1.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTGTTTTAGTTATTGG |
| Rev primer name |
SPAC15E1.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTGGTTTTCTGTACTGC |
| Amino acid length |
187 |
| Molecular weight |
20.5 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSEKFRQLLI/LKHVCSDLKL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |