| Gene name |
SPBC28F2.12 |
| Gene ID |
41/B01 |
| Gene synonyms/obsolete |
rpb1 |
| Gene product |
DNA-directed RNA
polymerase II (largest subunit); involved in transcription
from Pol II promoter; DNA-directed RNA polymerase activity
(function of complex); DNA binding activity; interacts
physically with Rpb5p |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
5557 |
| ORF length (spliced) |
5259 |
| Entry clone length |
5557 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC28F2.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCGGGATTCAGGTATG |
| Rev primer name |
SPBC28F2.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGATGGGCTAGTAGGGGAA |
| Amino acid length |
1752 |
| Molecular weight |
194.1 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNELIAKLTI |
| Localization (YFP) |
nucleus>cytosol;
cytosol |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |