| Gene name |
SPBC19C7.03 |
| Gene ID |
41/A12 |
| Gene synonyms/obsolete |
cyr1; git2 |
| Gene product |
adenylate
cyclase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
5079 |
| ORF length (spliced) |
|
| Entry clone length |
5079 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1089T:C / 1914A:G /
4192T:deletion / 5073T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC19C7.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCAAAGCAAGCGATT |
| Rev primer name |
SPBC19C7.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCCAGTGTGTCCAAGCATT |
| Amino acid length |
1692 |
| Molecular weight |
190.3 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVNLTYLDL/LVKLKHLYL/LKHLVHLDL/LTRTISQLKI/LSKSLRFLDI/LPQLEYLNL/LKNLKFLSI/LLWCFSVQLQL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |