| Gene name |
SPBC25H2.13c |
| Gene ID |
41/B02 |
| Gene synonyms/obsolete |
cdc20; pol2 |
| Gene product |
DNA polymerase epsilon
(catalytic subunit a); involved in telomere maintenance |
| Entry clone |
Cloned#/ 3' FS |
| ORF length (unspliced) |
6600 |
| ORF length (spliced) |
|
| Entry clone length |
6600 |
| No. of intron |
0 |
| Sequence status |
planning for
re-cloning |
| Sequence results |
6512T:C /
6584T:deletion |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC25H2.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCTTAAAAACAGCTCG |
| Rev primer name |
SPBC25H2.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTCAGCACAGAAAGTATG |
| Amino acid length |
2199 |
| Molecular weight |
252.8 |
| Isoelectric point (calc.) |
7.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLKSIKTLPL/LKKSLLSLLQI/LVDNLYHQLTL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |