Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC24H6.05
Gene ID 40/F12
Gene synonyms/obsolete cdc25; sal2
Gene product M-phase inducer phosphatase; involved in G2/M phase transition; involved in cell cycle checkpoint; involved in DNA damage checkpoint; involved in DNA repair checkpoint; involved in the regulation of CDK activity; involved in control of mitosis
Entry clone Cloned
ORF length (unspliced) 1791
ORF length (spliced)
Entry clone length 1791
No. of intron 0
Sequence status Finished
Sequence results 25T:C / 1557T:C / 1779T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC24H6.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTCTCCGCTTTCTTC
Rev primer name SPAC24H6.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATCTTCTAAGTGTAGAG
Amino acid length 596
Molecular weight 66.5
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: nucleus>cytosol
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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