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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1734.02c
Gene ID 40/E06
Gene synonyms/obsolete cdc27; SPBC337.18c
Gene product DNA polymerase (delta subunit); involved in G2/M phase transition (required); interacts physically with Pcn1p; recruits PCNA to the Pol delta holoenzyme; interacts physically with Cdc1p; essential; involved in DNA replication (required); involved in telomere maintenance
Entry clone Cloned
ORF length (unspliced) 1408
ORF length (spliced) 1119
Entry clone length 1408
No. of intron 5
Sequence status Finished
Sequence results 6G:deletion / 7G:deletion / 8A:deletion
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1734.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGAATGGAGAAACTT
Rev primer name SPBC1734.02.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCTTTCCAAAAAAGGAC
Amino acid length 372
Molecular weight 42.3
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 294
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVTVDNLSL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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