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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4D7.05
Gene ID 40/E05
Gene synonyms/obsolete sum1; tif34
Gene product translation initiation factor 3 (eif3 subunit 2); WD repeat protein; overexpression suppresses S/M checkpoint mutants; essential; involved in translation initiation (required); inhibits osmotic stress cell cycle; overexpression results in reduced global translation; interacts physically with Int6p; interacts physically with Mts2p; interacts physically with Mts4p; colocalizes stably with the 26S proteasome at the nuclear periphery; localization 26S proteasome dependent
Entry clone Cloned
ORF length (unspliced) 1398
ORF length (spliced) 987
Entry clone length 1398
No. of intron 2
Sequence status Finished
Sequence results 1017T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4D7.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGGCCTATCATTTTACA
Rev primer name SPAC4D7.05.Rv
Rev primer SEQ AGAAAGCTGGGTACAATGTATACTTGAAGTCA
Amino acid length 328
Molecular weight 36.8
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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