| Gene name |
SPBC19C2.05 |
| Gene ID |
40/E07 |
| Gene synonyms/obsolete |
ran1; pat1 |
| Gene product |
serine/threonine
protein kinase; involved in conjugation (regulation)
(negative); involved in meiosis (regulation) (negative);
involved in protein amino acid phosphorylation (of
Mei2p) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1413 |
| ORF length (spliced) |
|
| Entry clone length |
1413 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
464T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC19C2.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGCGCGAAAATCCAGA |
| Rev primer name |
SPBC19C2.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGCGATTTTCGAGATGGA |
| Amino acid length |
470 |
| Molecular weight |
52.2 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots;
nucleus>cytosol; cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |