Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19C2.05
Gene ID 40/E07
Gene synonyms/obsolete ran1; pat1
Gene product serine/threonine protein kinase; involved in conjugation (regulation) (negative); involved in meiosis (regulation) (negative); involved in protein amino acid phosphorylation (of Mei2p)
Entry clone Cloned
ORF length (unspliced) 1413
ORF length (spliced)
Entry clone length 1413
No. of intron 0
Sequence status Finished
Sequence results 464T:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19C2.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGCGCGAAAATCCAGA
Rev primer name SPBC19C2.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGCGATTTTCGAGATGGA
Amino acid length 470
Molecular weight 52.2
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus>cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.