| Gene name |
SPCC550.03c |
| Gene ID |
39/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
putative DEAD/DEAH box
helicase DEAD/DEAH box helicase; Sc SKI2 is part of a system
to protect cells from double-stranded RNA (dsRNA)
viruses |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3642 |
| ORF length (spliced) |
|
| Entry clone length |
3642 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
534A:G / 543C:T /
597A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC550.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCTAAACTTGTAGA |
| Rev primer name |
SPCC550.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATATACAGTGAAGGGCAG |
| Amino acid length |
1213 |
| Molecular weight |
138 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLRVETLRI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |