Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1D4.12
Gene ID 38/C06
Gene synonyms/obsolete rhp3; rad15
Gene product transcription initiation factor TFIIH subunit; involved in DNA repair (required); involved in nucleotide-excision repair; DEAD/DEAH box helicaseXP-D family; functionally complements Sc RAD3; functionally complemented by Sc RAD3
Entry clone Cloned
ORF length (unspliced) 2319
ORF length (spliced)
Entry clone length 2319
No. of intron 0
Sequence status Finished
Sequence results 174A:G / 1758T:A / 1879A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1D4.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGTTCTATATTGACGA
Rev primer name SPAC1D4.12.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTTTCAACCACATCAATA
Amino acid length 772
Molecular weight 88.1
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTSLVRALQI/LVAFFPSYLYL
Localization (YFP) nucleus>cytosol
Comments for localization a few cytoplasmic dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.