Gene name |
SPAC3H1.04c |
Gene ID |
37/A01 |
Gene synonyms/obsolete |
|
Gene product |
conserved fungal
protein |
Entry clone |
Cloned#/Sequence
mismatch |
ORF length (unspliced) |
1907 |
ORF length (spliced) |
1806 |
Entry clone length |
1907 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
1684T:addition |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC3H1.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCGAATCACGACTTGC |
Rev primer name |
SPAC3H1.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCGAAATTACTGATTGAA |
Amino acid length |
601 |
Molecular weight |
69.4 |
Isoelectric point (calc.) |
10.3 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRRFLHLVL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |