Gene name |
SPCC1840.08c |
Gene ID |
35/C04 |
Gene synonyms/obsolete |
|
Gene product |
glycoprotein;
thioredoxin related glycoprotein; similar to Sp SPAC959.05C
and SPBC3D6.13C |
Entry clone |
Cloned |
ORF length (unspliced) |
1686 |
ORF length (spliced) |
|
Entry clone length |
1686 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1840.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGCTGGGTGCTTGGAG |
Rev primer name |
SPCC1840.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTTGCTTTAACAAAGAG |
Amino acid length |
561 |
Molecular weight |
64.7 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDTTFRVLAI/LISLWISLIL |
Localization (YFP) |
ER; Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |