| Gene name |
SPBC1A4.03c |
| Gene ID |
34/B05 |
| Gene synonyms/obsolete |
top2 |
| Gene product |
DNA topoisomerase II;
DNA gyrase B domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4514 |
| ORF length (spliced) |
4458 |
| Entry clone length |
4514 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
141A:G / 513A:G /
4503G:A |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1A4.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCATTGATGCGGATTT |
| Rev primer name |
SPBC1A4.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCACTCTCATCATAATCG |
| Amino acid length |
1485 |
| Molecular weight |
167.8 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
1225 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus as
dots>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |