| Gene name |
SPBC16D10.04c |
| Gene ID |
34/B04 |
| Gene synonyms/obsolete |
dna2 |
| Gene product |
DNA helicase; involved
in DNA repair; involved in DNA replication; essential;
involved in Okazaki fragment processing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4334 |
| ORF length (spliced) |
4197 |
| Entry clone length |
4334 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
1208A:G / 1690A:G /
2850T:C / 3985A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16D10.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCAACGACCAGTCGAA |
| Rev primer name |
SPBC16D10.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATTCCAGTTGAGGTAAA |
| Amino acid length |
1398 |
| Molecular weight |
157.7 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPICLGPLQL |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |