Gene name |
SPBC27B12.04c |
Gene ID |
33/H01 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
2454 |
ORF length (spliced) |
|
Entry clone length |
2454 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1841A:G /
2294T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC27B12.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGCTACAACGGCTAG |
Rev primer name |
SPBC27B12.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCAATGTCATTTATGTGC |
Amino acid length |
817 |
Molecular weight |
95 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSLMFFFLTL/LPTLVKLFI/LYSLSSLFL/LHENLKKLLAI/LYALKVLKL |
Localization (YFP) |
vacuole
membrane? |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |