Gene name |
SPBC27B12.12c |
Gene ID |
33/G12 |
Gene synonyms/obsolete |
|
Gene product |
metal ion transporter;
MIT family; inorganic cation transporter |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
2412 |
ORF length (spliced) |
|
Entry clone length |
2412 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC27B12.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTTCAACAACCTTC |
Rev primer name |
SPBC27B12.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGGCATTGTATCGCATA |
Amino acid length |
803 |
Molecular weight |
89.3 |
Isoelectric point (calc.) |
6.1 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
104/106/108/110 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |