Gene name |
SPBC25D12.05 |
Gene ID |
33/C08 |
Gene synonyms/obsolete |
trm1 |
Gene product |
N2,N2-dimethylguanosine tRNA methyltransferase |
Entry clone |
Cloned in 2004
trial_also cloned#/ 3' FS at 1st |
ORF length (unspliced) |
1647 |
ORF length (spliced) |
|
Entry clone length |
1647 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC25D12.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATGAAGCCAATATGCT |
Rev primer name |
SPBC25D12.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGATTTTGAGTCAACTTCA |
Amino acid length |
548 |
Molecular weight |
60.2 |
Isoelectric point (calc.) |
8.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion;
nucleus |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |