| Gene name |
SPAC1805.05 |
| Gene ID |
33/B03 |
| Gene synonyms/obsolete |
cki3 |
| Gene product |
serine/threonine
protein kinase; casein kinase; similar to Sp SPBC1347.06c and
SPBP35G2.05c (paralogs) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1519 |
| ORF length (spliced) |
1320 |
| Entry clone length |
1519 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
239A:G / 1514G:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1805.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAACTACTTCGTCTCA |
| Rev primer name |
SPAC1805.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTTGTTTCGATATGCAG |
| Amino acid length |
439 |
| Molecular weight |
50.2 |
| Isoelectric point (calc.) |
9.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSLFDSLLL |
| Localization (YFP) |
periphery, especially
at site of septum formation |
| Comments for localization |
almost no apparent
signal |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |