Gene name |
SPBC543.06c |
Gene ID |
33/B02 |
Gene synonyms/obsolete |
|
Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; involved in ribosome
biogenesis and assembly |
Entry clone |
Cloned |
ORF length (unspliced) |
1518 |
ORF length (spliced) |
1362 |
Entry clone length |
1518 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC543.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAACATACAAGAAA |
Rev primer name |
SPBC543.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTTTCCGTAGATAAAGTA |
Amino acid length |
453 |
Molecular weight |
50.7 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIDTLKALAI/LIYSILRLLEL |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |