Gene name |
SPBC1539.03c |
Gene ID |
32/H10 |
Gene synonyms/obsolete |
argx |
Gene product |
argininosuccinate
lyase (putative); involved in arginine metabolism; arginine
biosynthesis pathway (final step) |
Entry clone |
Cloned |
ORF length (unspliced) |
1383 |
ORF length (spliced) |
|
Entry clone length |
1383 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
191A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1539.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCTTCACAAAAACT |
Rev primer name |
SPBC1539.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCCAAGGCCTTTTTAATG |
Amino acid length |
460 |
Molecular weight |
51.5 |
Isoelectric point (calc.) |
5.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |