Gene name |
SPBC646.05c |
Gene ID |
32/H09 |
Gene synonyms/obsolete |
erg9 |
Gene product |
squalene synthase;
farnesyl-diphosphate farnesyltransferase; involved in
ergosterol biosynthesis |
Entry clone |
Cloned |
ORF length (unspliced) |
1383 |
ORF length (spliced) |
|
Entry clone length |
1383 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC646.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTTAGCTAACCGCAT |
Rev primer name |
SPBC646.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAACAAATTAAGCTCTTTG |
Amino acid length |
460 |
Molecular weight |
53.3 |
Isoelectric point (calc.) |
5.5 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKNVCDLFL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |