| Gene name |
SPBC16E9.17c |
| Gene ID |
32/G07 |
| Gene synonyms/obsolete |
rem1 |
| Gene product |
B-type cyclin;
essential for meiotic progression; overexpression results in
cut phenotype |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1294 |
| ORF length (spliced) |
1209 |
| Entry clone length |
1294 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
780A:G / 1028A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16E9.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACTCTAACAACAAAAG |
| Rev primer name |
SPBC16E9.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTACCATCCATCGCCATC |
| Amino acid length |
402 |
| Molecular weight |
46 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSHMEKLEI |
| Localization (YFP) |
nucleus>cytosol;
nuclear envelope? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |