Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23G3.03
Gene ID 32/G08
Gene synonyms/obsolete
Gene product peptide monooxygenase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1296
ORF length (spliced)
Entry clone length 1296
No. of intron 0
Sequence status Finished
Sequence results 1270C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23G3.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGAACCTCTAGATTT
Rev primer name SPAC23G3.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAAGATAAGAAGCAGGG
Amino acid length 431
Molecular weight 48.5
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; cytoplasmic dots; filamentous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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