Gene name |
SPBC4.05 |
Gene ID |
32/C04 |
Gene synonyms/obsolete |
mlo2 |
Gene product |
zinc finger protein;
zf-PHD finger; involved in transcriptional regulation; no
apparent Sc ortholog; similar to D. melanogaster CG15141;
non-essential |
Entry clone |
Cloned# |
ORF length (unspliced) |
990 |
ORF length (spliced) |
|
Entry clone length |
990 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC4.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGGAAACGGCTCATGA |
Rev primer name |
SPBC4.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCGTAAATTTTTACTGATT |
Amino acid length |
329 |
Molecular weight |
38 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |