| Gene name |
SPBC27B12.03c |
| Gene ID |
32/C05 |
| Gene synonyms/obsolete |
|
| Gene product |
C-5 sterol desaturase;
involved in ergosterol biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
990 |
| ORF length (spliced) |
|
| Entry clone length |
990 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC27B12.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGTCGTTTTGCAGTA |
| Rev primer name |
SPBC27B12.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTATCCTTCTTGCTGGCA |
| Amino acid length |
329 |
| Molecular weight |
39 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
4 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER; Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |