Gene name |
SPBC4F6.15c |
Gene ID |
32/C03 |
Gene synonyms/obsolete |
swi10 |
Gene product |
endonuclease complex
subunit; helix-hairpin-helix; involved in mating-type
determination, mismatch repair, nucleotide-excision repair,
DNA repair, strand incision |
Entry clone |
Cloned |
ORF length (unspliced) |
989 |
ORF length (spliced) |
759 |
Entry clone length |
989 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC4F6.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGATATCGATGATGA |
Rev primer name |
SPBC4F6.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTTTGTTTTAAATTTATT |
Amino acid length |
252 |
Molecular weight |
28.6 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |