Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC36B7.08c
Gene ID 32/C02
Gene synonyms/obsolete
Gene product involved in chromatin decondensation; template activating factor; involved in nucleosome assembly; similar to human oncoprotein SET (TAF1beta)
Entry clone Cloned
ORF length (unspliced) 987
ORF length (spliced) 735
Entry clone length 987
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC36B7.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGCAGCTCAAGCTTT
Rev primer name SPBC36B7.08.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAGACTTCTTGGACTTT
Amino acid length 244
Molecular weight 28.6
Isoelectric point (calc.) 4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica

Image information
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