Gene name |
SPBC25B2.05 |
Gene ID |
32/C01 |
Gene synonyms/obsolete |
mis3 |
Gene product |
RNA-binding protein;
KH domain; involved in ribosome biogenesis and assembly |
Entry clone |
Cloned# |
ORF length (unspliced) |
984 |
ORF length (spliced) |
|
Entry clone length |
984 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC25B2.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGAAGTAGAAAGTTC |
Rev primer name |
SPBC25B2.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCCCTTTTACGCTTTTTA |
Amino acid length |
327 |
Molecular weight |
38.5 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
288 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |