| Gene name |
SPAC16E8.01 |
| Gene ID |
30/G12 |
| Gene synonyms/obsolete |
|
| Gene product |
actin cortical patch
component; involved actin cytoskeletal organization; src (SH3)
homology domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4409 |
| ORF length (spliced) |
4263 |
| Entry clone length |
4409 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
606A:G / 2302A:G /
3261T:C / 3584T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16E8.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAAATTTACCTATAAT |
| Rev primer name |
SPAC16E8.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAACCAAAAGGATTGTCT |
| Amino acid length |
1420 |
| Molecular weight |
154.6 |
| Isoelectric point (calc.) |
5.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |