| Gene name |
SPBC211.03c |
| Gene ID |
30/G11 |
| Gene synonyms/obsolete |
|
| Gene product |
guanyl-nucleotide
exchange factor activity; Sec7 domain; similar to S.
cerevisiae YJR031C and YEL022W |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4389 |
| ORF length (spliced) |
|
| Entry clone length |
4389 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
546A:G / 2547A:G /
2874T:C / 3932T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC211.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGTGCCTCGCGCATC |
| Rev primer name |
SPBC211.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACAGTAGTCGTTTGTGAG |
| Amino acid length |
1462 |
| Molecular weight |
165.4 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLQAMPGSLKL/LHKVVSSLDL/LNKLLDSLLSL |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
bright dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |