| Gene name |
SPCC1682.15 |
| Gene ID |
28/B01 |
| Gene synonyms/obsolete |
|
| Gene product |
PXA domain; PX domain;
involved in intracellular signaling cascade; no apparent
orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2250 |
| ORF length (spliced) |
|
| Entry clone length |
2250 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
462T:C / 1941A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1682.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATCGAAAATGGGATTT |
| Rev primer name |
SPCC1682.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATGATGCTACGAAGAGCA |
| Amino acid length |
749 |
| Molecular weight |
85.6 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLIFYILYI/LPPELEAPLQL/LVGDLLTSLIL |
| Localization (YFP) |
ER with
discontinuity |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |