Gene name |
SPCC1682.15 |
Gene ID |
28/B01 |
Gene synonyms/obsolete |
|
Gene product |
PXA domain; PX domain;
involved in intracellular signaling cascade; no apparent
orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
2250 |
ORF length (spliced) |
|
Entry clone length |
2250 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
462T:C / 1941A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1682.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATCGAAAATGGGATTT |
Rev primer name |
SPCC1682.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATGATGCTACGAAGAGCA |
Amino acid length |
749 |
Molecular weight |
85.6 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLIFYILYI/LPPELEAPLQL/LVGDLLTSLIL |
Localization (YFP) |
ER with
discontinuity |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |