Gene name |
SPBC25H2.16c |
Gene ID |
27/F01 |
Gene synonyms/obsolete |
|
Gene product |
VHS domain; GAT
domain; involved in intracellular protein transport; adaptin
C-terminal domain; similar to S. pombe SPAC1F3.05 |
Entry clone |
Cloned |
ORF length (unspliced) |
2041 |
ORF length (spliced) |
1602 |
Entry clone length |
2041 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC25H2.16.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAGCGCTAAAAGAAG |
Rev primer name |
SPBC25H2.16.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAGTAGCCTAATTGAAGAT |
Amino acid length |
533 |
Molecular weight |
59.4 |
Isoelectric point (calc.) |
5.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |