Gene name |
SPBC3D6.03c |
Gene ID |
27/E12 |
Gene synonyms/obsolete |
|
Gene product |
metallo-beta-lactamase
family protein; similar to Sp SPAC1D4.10 |
Entry clone |
Cloned |
ORF length (unspliced) |
2037 |
ORF length (spliced) |
|
Entry clone length |
2037 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
899T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3D6.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGCTTCTCTTCTGGT |
Rev primer name |
SPBC3D6.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATCGTTTCTCGCTGCTTG |
Amino acid length |
678 |
Molecular weight |
75.9 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
mitochondrion; periphery at site of septum formation |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |