| Gene name |
SPAC1B3.04c |
| Gene ID |
27/E11 |
| Gene synonyms/obsolete |
|
| Gene product |
GTP binding protein;
Elongation factor Tu family |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2029 |
| ORF length (spliced) |
1941 |
| Entry clone length |
2029 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1291T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1B3.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTATTTTTAGGCTCTC |
| Rev primer name |
SPAC1B3.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGCAATGTAATTACTTACC |
| Amino acid length |
646 |
| Molecular weight |
72.6 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNDNIEQLAL/LGFLGTLHL/LCSIVHRSLAL |
| Localization (YFP) |
periphery;
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|