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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F7.10
Gene ID 26/H01
Gene synonyms/obsolete
Gene product palmitoyltransferase activity; 6 ankyrin repeat protein; zinc finger protein; zf-DHHC type; AKR/ZDHHC17 subfamily; palmitoyltransferase specific for casein kinase 1 (By similarity)
Entry clone Cloned#
ORF length (unspliced) 1929
ORF length (spliced)
Entry clone length 1929
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC2F7.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTTCATTATTTTTAGC
Rev primer name SPAC2F7.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAACATGTCTTGTTGCCTCA
Amino acid length 642
Molecular weight 72.5
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLVVYLLHL/LLYIVPKTLPI/LRTVCGILGL
Localization (YFP) Golgi; vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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