Gene name |
SPAC1B1.04c |
Gene ID |
26/H02 |
Gene synonyms/obsolete |
|
Gene product |
polyA specific
ribonuclease subunit |
Entry clone |
Cloned |
ORF length (unspliced) |
1929 |
ORF length (spliced) |
1815 |
Entry clone length |
1929 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
389G:A / 1215T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1B1.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATGCGACCTTGTTGAT |
Rev primer name |
SPAC1B1.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCAAGTTTTTTCAAAAATG |
Amino acid length |
604 |
Molecular weight |
67.7 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion;
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |