Gene name |
SPAC22G7.05 |
Gene ID |
26/G11 |
Gene synonyms/obsolete |
|
Gene product |
involved in ribosome
biogenesis and assembly; predicted coiled-coil protein;
interacts physically with Mis3p |
Entry clone |
Cloned |
ORF length (unspliced) |
1924 |
ORF length (spliced) |
1797 |
Entry clone length |
1924 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
319A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22G7.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCAGAAAAAAATCAGC |
Rev primer name |
SPAC22G7.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTCCCAAAAACTTGTTTC |
Amino acid length |
598 |
Molecular weight |
69.5 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB?; nucleus |
Comments for localization |
one nucleolar dot by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |