Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4F10.15c
Gene ID 26/G03
Gene synonyms/obsolete wsp1
Gene product Wiskott-Aldrich homolog; involved in stress response; involved in cytokinesis; involved in actin cytoskeletal organization; involved in control of the actin cytoskeleton; actin cortical patch component; involved in actin cortical patch assembly; involved in actin cortical patch distribution; promoter homolD box; involved in endocytosis
Entry clone Cloned
ORF length (unspliced) 1919
ORF length (spliced) 1725
Entry clone length 1919
No. of intron 3
Sequence status Finished
Sequence results 1275G:A / 1585T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4F10.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCCATCTTCCTCTAT
Rev primer name SPAC4F10.15.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCCCACTCATCGTCGTCT
Amino acid length 574
Molecular weight 59.6
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation DeltaVision

Image information
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