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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC613.12c
Gene ID 26/G02
Gene synonyms/obsolete
Gene product WD repeat protein; similar to N.crassa B13I18.130; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1917
ORF length (spliced)
Entry clone length 1917
No. of intron 0
Sequence status Finished
Sequence results 50T:C / 974T:C / 1108A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC613.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTAATAGTTCACCACG
Rev primer name SPCC613.12.Rv
Rev primer SEQ AGAAAGCTGGGTAGGTTAAAAGTCGATTTTCT
Amino acid length 638
Molecular weight 71.5
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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