Gene name |
SPAC1B3.15c |
Gene ID |
26/D09 |
Gene synonyms/obsolete |
|
Gene product |
transporter;
localization membrane; unknown specificity; allantoate
permease family; tandem duplication; similar to Sp SPAC1B3.16c
(paralog) |
Entry clone |
Cloned# |
ORF length (unspliced) |
1887 |
ORF length (spliced) |
|
Entry clone length |
1887 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1B3.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACACAAACACCTTACC |
Rev primer name |
SPAC1B3.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTTACAGATAACTCTTTC |
Amino acid length |
628 |
Molecular weight |
69 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
|
No. of transmembrane domain |
11 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSRIRRKLDL/LRFFNGLAI/LFVLAGLLI |
Localization (YFP) |
Golgi; periphery,
especially at cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |