Gene name |
SPCC622.11 |
Gene ID |
26/D08 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein; conserved in C. elegans,
D. melanogaster CG8135 and A. thaliana; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1884 |
ORF length (spliced) |
1689 |
Entry clone length |
1884 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC622.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCAGTTGGTTCAATGT |
Rev primer name |
SPCC622.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACATGGGCATTATTTGTA |
Amino acid length |
562 |
Molecular weight |
64.8 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
|
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIEMLGLLLLL/LYVPFVVTLLI |
Localization (YFP) |
Golgi; cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |