| Gene name |
SPBC16D10.07c |
| Gene ID |
26/D07 |
| Gene synonyms/obsolete |
|
| Gene product |
transcriptional
regulator; Sir2p family; histone deacetylase activity;
NAD-dependent histone deacetylase activity; involved in
transcriptional regulation; involved in chromatin silencing;
involved in chromatin silencing at silent mating type
cassettes (sensu Fungi) (required); involved in chromatin
silencing at centromere (required); involved in chromatin
silencing at telomere (required); involved in chromatin
assembly/disassembly |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1884 |
| ORF length (spliced) |
1416 |
| Entry clone length |
1884 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC16D10.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCGACGACTCCCGTTGA |
| Rev primer name |
SPBC16D10.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACAACTACCGTTTGTTGTG |
| Amino acid length |
471 |
| Molecular weight |
53 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |