| Gene name |
SPBC14F5.11c |
| Gene ID |
26/D06 |
| Gene synonyms/obsolete |
|
| Gene product |
PX domain;
phosphoinositide binding; involved in intracellular protein
transport |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1879 |
| ORF length (spliced) |
1761 |
| Entry clone length |
1879 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
125A:G / 334A:G /
706T:C / 808T:C / 1740T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC14F5.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTTTTTGAAGATAA |
| Rev primer name |
SPBC14F5.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGATACATATGTTTTCAAT |
| Amino acid length |
586 |
| Molecular weight |
67 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGAMFNALSL |
| Localization (YFP) |
cytosol |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |