| Gene name |
SPCC757.12 |
| Gene ID |
26/D05 |
| Gene synonyms/obsolete |
|
| Gene product |
glycosyl hydrolase
family 13; alpha-amylase; GPI anchored protein(pers. comm.
Birgit Eisenhaber); glycoprotein; no apparent Sc
ortholog |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1878 |
| ORF length (spliced) |
|
| Entry clone length |
1878 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC757.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGTTTTCTAAAATAGC |
| Rev primer name |
SPCC757.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGAATAGAACGATAGCA |
| Amino acid length |
625 |
| Molecular weight |
68 |
| Isoelectric point (calc.) |
4.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |