| Gene name |
SPBC2G5.08 |
| Gene ID |
26/D02 |
| Gene synonyms/obsolete |
SPBC25B2.01 |
| Gene product |
elongation factor 1
alpha |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1876 |
| ORF length (spliced) |
1779 |
| Entry clone length |
1876 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC2G5.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAGGCATCGTGATGT |
| Rev primer name |
SPBC2G5.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACATAATTCTTTAACAATG |
| Amino acid length |
592 |
| Molecular weight |
66 |
| Isoelectric point (calc.) |
5.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDGLFPLCL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
| Microscope used for
observation |
DeltaVision |