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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1071.06
Gene ID 25/D02
Gene synonyms/obsolete
Gene product actin-like protein; SWI-SNF global transcription activator complex; involved in chromatin remodeling
Entry clone Cloned
ORF length (unspliced) 1572
ORF length (spliced)
Entry clone length 1572
No. of intron 0
Sequence status Finished
Sequence results 871T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1071.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTTCTTTCAGGGATGA
Rev primer name SPAC1071.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTGCTTTGTATGAATCGCT
Amino acid length 523
Molecular weight 59.1
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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