| Gene name |
SPBC3D6.07 |
| Gene ID |
25/D03 |
| Gene synonyms/obsolete |
|
| Gene product |
transferase activity,
transferring hexosyl groups; glycosyl transferases group 1;
involved in GPI anchor biosynthesis (early step) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1572 |
| ORF length (spliced) |
1371 |
| Entry clone length |
1572 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
849T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3D6.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATCAGACTTCTTCTT |
| Rev primer name |
SPBC3D6.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGTGCATCTTTTAAACAT |
| Amino acid length |
456 |
| Molecular weight |
51.7 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRLIDRLKL |
| Localization (YFP) |
ER; cytoplasmic
dots |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |