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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC17D11.08
Gene ID 25/D01
Gene synonyms/obsolete
Gene product conserved hypothetical; WD repeat protein
Entry clone Cloned
ORF length (unspliced) 1571
ORF length (spliced) 1308
Entry clone length 1571
No. of intron 4
Sequence status Finished
Sequence results 1418T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC17D11.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAGATTTACGTAGAAA
Rev primer name SPBC17D11.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTGAGGGACCTTCAGTATT
Amino acid length 435
Molecular weight 47.7
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi?; cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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