Gene name |
SPBC17D11.08 |
Gene ID |
25/D01 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; WD repeat protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1571 |
ORF length (spliced) |
1308 |
Entry clone length |
1571 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
1418T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC17D11.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAGATTTACGTAGAAA |
Rev primer name |
SPBC17D11.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGAGGGACCTTCAGTATT |
Amino acid length |
435 |
Molecular weight |
47.7 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi?; cytosol;
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |